A DryLab® AQbD approach to facilitate pH-interpretation in RPC

A recent collaboration involving scientists from the MOLNÁR-INSTITUTE for Applied Chromatography has achieved groundbreaking results in developing clear modelling strategies to address the longstanding challenge of selecting appropriate pH modifiers for robust separation of ionizable compounds in high-performance liquid chromatography (HPLC). The team, comprising experts from both industry and academia, focused on one of the most common practical issues in reversed-phase chromatography (RPC): the substitution of an equimolar non-volatile phosphate buffer with a volatile acetate buffer within the same nominal pH range.

The study Extended multidimensional design space studies: Comparing volatile and non-volatile buffer systems in UPLC was recently accepted for publication in the peer-reviewed Journal of Chromatography A. The research was supervised and actively supported by Dr. Imre Molnár, President of the Molnár-Institute, in collaboration with Professor Krisztián Horváth from the University of Pannonia and renowned industry expert Dr. Róbert Kormány of Budapest-based Egis Pharmaceuticals.